Analysis of genetic variability in F2 interspecific hybrids of mung bean (Vigna radiata) using inter-retrotransposon amplified polymorphism marker system

Abstract. Fatmawati Y, Setiawan AB, Purwantoro A, Respatie DW, Teo CH. 2021. Analysis of genetic variability in F2 interspecific hybrids mung bean (Vigna radiata) using inter-retrotransposon amplified polymorphism marker system. Biodiversitas 22: 4880-4889. Mung radiata L. Wilczek) categorized as one the pivotal annual crops Vigna genera is commonly cultivated rotation with cereal during drought season. Conversely, to ameliorate its stunted productivity, hybridization technique has been introduced between and common promote improvement breeding projects Indonesia. However, since a self-pollinated crop narrow base, selection specific trait marker-assisted more challenging. Hence, precautionary investigation imperative evaluate progenies resulting from an ideal marker. This study aimed investigate population retrotransposon-based markers, particularly Inter-Retrotransposon Amplified Polymorphism (IRAP) markers. In this study, we identified retrotransposon genome determined Long Terminal Repeat (LTR) sequence LTR Finder. The IRAP primers were designed conserved region sequence. One hundred generated crossing successfully discriminated by showed high heterozygosity moderate Polymorphic Information Content (PIC) values. markers able detect hybridization. Cluster analysis that 100 grouped into three clusters. demonstrated can offer effective approach for evaluating segregation intercross bean.